منابع مشابه
Cas9 Functionally Opens Chromatin
Using a nuclease-dead Cas9 mutant, we show that Cas9 reproducibly induces chromatin accessibility at previously inaccessible genomic loci. Cas9 chromatin opening is sufficient to enable adjacent binding and transcriptional activation by the settler transcription factor retinoic acid receptor at previously unbound motifs. Thus, we demonstrate a new use for Cas9 in increasing surrounding chromati...
متن کاملRearranging the chromatin for pluripotency
Pluripotent cells are characterized by infinite self-renewal and unrestricted differentiation potential with far-reaching implications in developmental biology and regenerative medicine. Somatic cells can be reprogrammed to a pluripotent state by ectopic expression of defined transcription factors (TFs) such as Oct4, Klf4, Sox2, and c-Myc, generating induced pluripotent stem cells (iPSCs). This...
متن کاملOpen conformation chromatin and pluripotency.
Pluripotency, the ability of a cell to give rise to all of the cells of an organism, is a fascinating and mysterious characteristic of embryonic stem (ES) cells. Recent extensive molecular studies of mouse ES cells have revealed the roles played by transcription factor networks and epigenetic processes in the maintenance of ES cell pluripotency (Niwa 2007). However, no direct evidence to connec...
متن کاملChromatin regulatory mechanisms in pluripotency.
Stem cells of all types are characterized by a stable, heritable state permissive of multiple developmental pathways. The past five years have seen remarkable advances in understanding these heritable states and the ways that they are initiated or terminated. Transcription factors that bind directly to DNA and have sufficiency roles have been most easy to investigate and, perhaps for this reaso...
متن کاملChromatin Connections to Pluripotency and Cellular Reprogramming
The pluripotent state of embryonic stem cells (ESCs) provides a unique perspective on regulatory programs that govern self-renewal and differentiation and somatic cell reprogramming. Here, we review the highly connected protein and transcriptional networks that maintain pluripotency and how they are intertwined with factors that affect chromatin structure and function. The complex interrelation...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science
سال: 2016
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.352.6289.1072-b